Human 3-Nitrotyrosine (3-NT) ELISA Kit

$690.00 $568.10
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FOR RESEARCH USE ONLY. Not for clinical DIAGNOSIS USE CATALOG #: 11631 INTRODUCTION  For the quantitative determination of Human 3-NT concentrations.  Detection of species: Human  Detection medium: serum, plasma, tissue homogenates, cell culture supernates

PRINCIPLE OF TEST
The kit is for the quantitative level of 3-NT in the sample, adopt purified Human 3-NT to coat microtiter plate, make
solid-phase antibody, then add 3-NT to wells, Combine 3-NT antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of 3-NT in the samples is then determined by comparing the O.D. of the samples
to the standard curve.

Elisa Kit for Research