Rat 5-Hydroxytryptamine(5HT) ELISA Kit

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Overview Product name: Rat 5-Hydroxytryptamine,5HT ELISA Kit Tests: 1 X 96 well plate,or 1 X 48 well plate Sample type: serum; Plasma; Cell culture supernatant Assay type: Sandwich Reacted Species Rat Product overview Rat 5-Hydroxytryptamine ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody […]

Overview

Product name: Rat 5-Hydroxytryptamine,5HT ELISA Kit
Tests: 1 X 96 well plate,or 1 X 48 well plate
Sample type: serum; Plasma; Cell culture supernatant
Assay type: Sandwich
Reacted Species Rat
Product overview Rat 5-Hydroxytryptamine ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 5-Hydroxytryptamine. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for 5-Hydroxytryptamine is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain c5-Hydroxytryptamine and HRP conjugated 5-Hydroxytryptamine antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 5-Hydroxytryptamine. You can calculate the concentration of 5-Hydroxytryptamine in the samples by comparing the OD of the samples to the standard curve.
Tested applications Sandwich ELISA

Properties

Storage Instructions l   The unopened kit shall be stored at [2-8 ℃] .l   For opened kit can be stored at [2-8 ℃] for up to 1 month. If not be used recently, the standard should be kept in -20 ℃.

 

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