Rat nuclear factor Kappa B (NFκb) ELISA Kit

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FOR RESEARCH USE ONLY. Not for clinical DIAGNOSIS USE CATALOG #:31239 INTRODUCTION  For the quantitative determination of Rat NFκ b concentrations.  Detection of species: Rat  Detection medium: serum, plasma, Urine, tissue samples, cell culture supernates

The kit is for the quantitative level of NFκ b in the sample, adopt purified Rat NFκ b to coat microtiter plate, make
solid-phase antibody, then add NFκ b to wells, Combine NFκ b antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue
color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is
measured at a wavelength of 450 nm. The concentration of NFκ b in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

Elisa Kit for Research