Human Elafin ELISA Kit


item no.12383


The kit is for the quantitative level of Elafin in the sample, adopt purified human Elafin
to coat microtiter plate, make solid-phase antibody, then add samples or standards to
wells with a labeled antibody specific to Elafin, then add labeled HRP to the well. After
washing completely, add TMB substrate solution, TMB substrate becomes blue color in
wells that contains antibody – antigen – enzyme-antibody complex, reaction is
terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of Elafin in the samples is then determined
by comparing the O.D. of the samples to the standard curve.


Elisa Kit for Research