Human silent information regulator 1 (Sirtuin1) ELISA Kit


For the quantitative determination of human Sirtuin1 concentrations.  Detection of species: Human  Detection medium: serum, plasma, tissue homogenates, cell culture supernates,urine

The kit is for the quantitative level of Sirtuin1 in the sample, adopt purified human Sirtuin1 to coat microtiter plate, make
solid-phase antibody, then add Sirtuin1 to wells, Combine Sirtuin1 antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color
at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of Sirtuin1 in the samples is then determined by comparing the O.D. of the
samples to the standard curve.

Elisa Kit for Research